11694 Ndufb11 Status: ES cell colonies screened / QC no positives
| Gene | Ndufb11 |
| Chromosome | X |
| Deletion Start | 20,617,257 |
| Deletion End | 20,615,378 |
| Deletion Size | 1,880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,617,103 | 20,617,123 | ACGAGAACCGACCATGCAGTG |
| TUP | 20,617,079 | 20,617,101 | CAGGAGGACCCAGAACCCGAGGA |
| TUR | 20,617,052 | 20,617,071 | CCTCTCACCTTCGCGTAGAC |
| TDF | 20,615,919 | 20,615,940 | GAGATTGGAGGGCGGATTAGAG |
| TDP | 20,615,891 | 20,615,918 | ACCAGGTCATGCAAGGCCAGGATATAAC |
| TDR | 20,615,847 | 20,615,870 | GAGGAGAAGGAGAGGTCAATTAGG |
PCR Primers
| Primer | Sequence |
| SU | AACGTTGATTCGGTAGCGAC |
| SD | ACTTAATAGCAACCACCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 322 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
