11695 5430427O19Rik Status: Cancelled
| Gene | 5430427O19Rik |
| Chromosome | X |
| Deletion Start | 85,887,833 |
| Deletion End | 85,888,726 |
| Deletion Size | 915 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,888,043 | 85,888,062 | GCGAGTCAGCACAGCAGAAG |
| TUP | 85,888,063 | 85,888,086 | CCAGAGCAGAACAGTGCTGCAGGC |
| TUR | 85,888,154 | 85,888,176 | GAAGGTGGAACCAAGTAGGTCTC |
| TDF | 85,888,392 | 85,888,414 | GGAGGATAACCAGCATCAAAGAG |
| TDP | 85,888,416 | 85,888,440 | AAAGCAGCCTGCAAATGCAGAAGCC |
| TDR | 85,888,469 | 85,888,490 | ATTCCACCACCTTCTGCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TACCTTTGTGCCTACCACTG |
| SD | CCTTCCTTCTCTCTGTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 390 bp | - | + | + |
| TUF | TUR | 134 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
