11697 Myo1g Status: ES cell colonies screened / QC no positives
| Gene | Myo1g |
| Chromosome | 11 |
| Deletion Start | 6,520,851 |
| Deletion End | 6,506,652 |
| Deletion Size | 14,200 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,520,050 | 6,520,068 | AGGTGCAGAGATGGAGGTC |
| TUP | 6,520,022 | 6,520,047 | AGGCTGCCACATGGAGAGCTTTATCC |
| TUR | 6,519,990 | 6,520,012 | GGCAGTCTAACAAAGCATTGAAC |
| TDF | 6,507,118 | 6,507,138 | CAGGTGGTGCTCAGGAAAGTC |
| TDP | 6,507,080 | 6,507,106 | AAGTGGTTGGCTTGATGTGACCCTGAC |
| TDR | 6,507,043 | 6,507,061 | TCACACTCAGCCCTGTCAC |
PCR Primers
| Primer | Sequence |
| SU | GTATGGCCAAACCTAGCTTG |
| SD | TGTCACTCACTTCAGGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 386 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
