11698 Ppdpf Status: Cancelled
| Gene | Ppdpf |
| Chromosome | 2 |
| Deletion Start | 181,187,668 |
| Deletion End | 181,188,301 |
| Deletion Size | 634 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,187,883 | 181,187,902 | CCGGGTAAGTGCAGAACCAG |
| TUP | 181,187,903 | 181,187,925 | CCAGCGGAAGGGCAGAGTTCACG |
| TUR | 181,187,954 | 181,187,974 | TCAAGTCCTTCTCCACGCTAC |
| TDF | 181,188,051 | 181,188,071 | GGGAAATCCACACTCCCGTTC |
| TDP | 181,188,076 | 181,188,102 | CTACAGTGTTGGAGTCTCCTGAGCGGT |
| TDR | 181,188,103 | 181,188,125 | ACAACCACATGGAAGATGGACTC |
PCR Primers
| Primer | Sequence |
| SU | AGTGACGAAGGCAGCCGG |
| SD | CAAGGCTGGATTCAGTAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
