11699 Cps1 Status: Cancelled
| Gene | Cps1 |
| Chromosome | 1 |
| Deletion Start | 67,123,168 |
| Deletion End | 67,152,366 |
| Deletion Size | 29,221 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 67,123,192 | 67,123,213 | GTGGTGAAGACCCTGAAGAGTG |
| TUP | 67,123,222 | 67,123,247 | TTTGCCAATGTGACTACGAAGCGACA |
| TUR | 67,123,254 | 67,123,273 | TGATGCCAGGTCTGGAGAAG |
| TDF | 67,151,470 | 67,151,494 | TCAGGCTGCATCTTTCATCTAATAC |
| TDP | 67,151,495 | 67,151,521 | TGTTGGCACACCATGGAGTCATTGTAA |
| TDR | 67,151,540 | 67,151,559 | TGGTCCTGGCTTCAAGAGTG |
PCR Primers
| Primer | Sequence |
| SU | ACAGGAGAAGGTGCCACTTG |
| SD | AGGACATAGTTGTCTGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 415 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 271 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
