11702 1110049F12Rik Status: ES cell colonies screened / QC no positives
| Gene | 1110049F12Rik |
| Chromosome | 4 |
| Deletion Start | 135,543,047 |
| Deletion End | 135,532,272 |
| Deletion Size | 10,776 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,542,347 | 135,542,364 | CCCATGCACCGCAGTTTG |
| TUP | 135,542,317 | 135,542,343 | AAAGTGATGCGGACGAAGAGCTCCTGT |
| TUR | 135,542,282 | 135,542,302 | AGGCAAGAGCCAAGGAGATAC |
| TDF | 135,532,427 | 135,532,446 | GTAGCCGCTGACCTCATCTC |
| TDP | 135,532,397 | 135,532,421 | TTTCCTGTAGCTTCGCCGGCATGAG
|
| TDR | 135,532,363 | 135,532,385 | GGTTGGCTGACGCTTCATAGTTG |
PCR Primers
| Primer | Sequence |
| SU | AAAGCGCACGCTTTGGCTC |
| SD | AGTGAGCATTATTCATGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 416 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
