11702   1110049F12Rik     Status: ES cell colonies screened / QC no positives

Gene1110049F12Rik
Chromosome4
Deletion Start135,987,132
Deletion End135,976,357
Deletion Size10,776
Genome Build38
MGI IDMGI:1913443
NCBI Gene ID66193
BAC ID54D1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,986,432135,986,449CCCATGCACCGCAGTTTG
TUP135,986,402135,986,428AAAGTGATGCGGACGAAGAGCTCCTGT
TUR135,986,367135,986,387AGGCAAGAGCCAAGGAGATAC
TDF135,976,512135,976,531GTAGCCGCTGACCTCATCTC
TDP135,976,482135,976,506TTTCCTGTAGCTTCGCCGGCATGAG
TDR135,976,448135,976,470GGTTGGCTGACGCTTCATAGTTG

PCR Primers

PrimerSequence
SUAAAGCGCACGCTTTGGCTC
SDAGTGAGCATTATTCATGACTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev416 bp-++
TUFTUR83 bp++-
TDFTDR84 bp++-
NeoFwdSD324 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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