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11704 Wbp7 Status: ES cell colonies screened / QC one positive

Gene	Wbp7
Chromosome	7
Deletion Start
Deletion End
Deletion Size
Genome Build	37

MGI ID	MGI:109565
NCBI Gene ID	75410
BAC ID	371M15
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	No

Sequence Downloads and Strategy Information

2,000 Bases Upstream (not yet available)
2,000 Bases Downstream (not yet available)
Primer Sequences
Cassette
Design Diagram
PCR Conditions
Genotyping Strategy

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	30,587,853	30,587,875	GCGGGTTGAAGAGGGATAGAATG
TUP	30,587,822	30,587,851	ATGCTGAGTCTTGAATCTGGAGTGAGTGCA
TUR	30,587,800	30,587,821	GCTGAGAGGGATAAACCATGAC
TDF	30,569,290	30,569,311	GGAAGGCCAGAAACACATTGTC
TDP	30,569,266	30,569,288	TCTTTGCCTTGCGCCGCATCCTG
TDR	30,569,218	30,569,239	GGCATCCTCGATAGGAAACTTG

PCR Primers

Primer	Sequence
SU	ACTGGTTCTCCTGGTCTCTG
SD	AGGAGGAAGGAGGCAATGG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	76 bp	+	+	-
TDF	TDR	94 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+