11706 Polg Status: Cancelled
| Gene | Polg |
| Chromosome | 7 |
| Deletion Start | 79,465,208 |
| Deletion End | 79,449,927 |
| Deletion Size | 15,282 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,464,456 | 79,464,474 | GGCTCGCTATGGTGGGAAG |
| TUP | 79,464,432 | 79,464,454 | TGCATAGGCTCCAGGCAACACCC |
| TUR | 79,464,382 | 79,464,405 | CTGTCTCAAACACAACACGGTATC |
| TDF | 79,452,534 | 79,452,556 | GCAATAGGTGACATGGTCTGGAG |
| TDP | 79,452,506 | 79,452,531 | CCGAGGAATGCTGAGAGTCTGGAACA |
| TDR | 79,452,480 | 79,452,501 | GCCAAGCCCAACTTCTTAACTC |
PCR Primers
| Primer | Sequence |
| SU | TTTGGCTAAGATCAAGTGGAG |
| SD | ATGCCTGAGTTAATGTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
