11708 1500001M20Rik Status: Targeting vector QC completed
| Gene | 1500001M20Rik |
| Chromosome | 6 |
| Deletion Start | 114,987,799 |
| Deletion End | 114,954,439 |
| Deletion Size | 33,361 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 114,987,504 | 114,987,523 | TTCGGTGTGAGCGGAATTTG |
| TUP | 114,987,478 | 114,987,501 | TTTCCCTGGTCAGGGTTGGCTGAA |
| TUR | 114,987,417 | 114,987,436 | GATTTGCCTGGCGTGACAAC |
| TDF | 114,954,613 | 114,954,633 | CACTGTAGCCCGAACCCAAAG |
| TDP | 114,954,589 | 114,954,611 | CAAGCCTCTGGGACGGTAGCTGC
|
| TDR | 114,954,554 | 114,954,573 | GCGTGGATGAGGAGTGAGTC |
PCR Primers
| Primer | Sequence |
| SU | CAGGTTGTCACAGGATAGAC |
| SD | CCTGAGTTAGTGTCTGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 60 bp | + | + | - |
| NeoFwd | SD | 385 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
