11714   Uchl1     Status: ES cell colonies screened / QC no positives

GeneUchl1
Chromosome5
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:103149
NCBI Gene ID22223
BAC ID231M6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF66,677,04166,677,059CCCTGCTGCTCCTGTTTCC
TUP66,677,06066,677,079CCTCACGGCCCAGGTAGGGA
TUR66,677,10766,677,128TCATTCCACAACACTGCGTATC
TDF66,686,37066,686,387AGGCTGCGGTTTACTGAC
TDP66,686,38866,686,410TCCTGGGCTGGGTGATTCTCTGA
TDR66,686,41366,686,435GGACTCAAAGGAAGGCAGAACTC

PCR Primers

PrimerSequence
SUCACAACCACCAGATTAGCTC
SDGGAAGACATTTGTCTGTGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR88 bp++-
TDFTDR66 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image