| Gene | 0610031J06Rik |
| Chromosome | 3 |
| Deletion Start | 88,325,085 |
| Deletion End | 88,328,387 |
| Deletion Size | 3,303 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,325,378 | 88,325,398 | GTCCTGCTTTCTGACGATGTC |
| TUP | 88,325,403 | 88,325,427 | CCATGGCTCAGCCATTGTAGCCCAG |
| TUR | 88,325,456 | 88,325,477 | CAGAGGGCTCAGAGCTAACAAG |
| TDF | 88,327,426 | 88,327,448 | CCTACCGAGTGCTGGGATTAAAG |
| TDP | 88,327,454 | 88,327,476 | CGCCATCACGCCTGGCTATGGTC |
| TDR | 88,327,534 | 88,327,554 | CACAGATGGCAGGCAATAGAC |
PCR Primers
| Primer | Sequence |
| SU | TTCACCAGAGGTGATGTAGG |
| SD | GGGAAATGTCTATTTCATGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 129 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11715A-A2 | VGB6 | C57BL/6NTac | |
| 11715A-H7 | VGB6 | C57BL/6NTac | |
| 11715A-E3 | VGB6 | C57BL/6NTac | |
| 11715A-H5 | VGB6 | C57BL/6NTac | |
| 11715A-F2 | VGB6 | C57BL/6NTac | |
