11719   Acbd4     Status: Cancelled

GeneAcbd4
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1914381
NCBI Gene ID67131
BAC ID121H4
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF103,104,050103,104,071CCTCATCTGTCACCCAGACTTC
TUP103,104,072103,104,095TCATGGACCTCTGCCTCCTAGGGA
TUR103,104,102103,104,122CCCTGCTCATCTTGCCTGTTC
TDF103,111,585103,111,605CCACGCTGCTCTTCTTTATCC
TDP103,111,606103,111,628TGTGGCCCTTCGTTGTCCAGTGG
TDR103,111,650103,111,671CGTCCTTGAGTCACCTCTTCTG

PCR Primers

PrimerSequence
SUGGTGTCTAGAGAGGTGCATC
SDGTCCCAACTTGCCCAAGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR73 bp++-
TDFTDR87 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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