11721   Nr0b1     Status: ES cell colonies screened / QC no positives

GeneNr0b1
ChromosomeX
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1352460
NCBI Gene ID11614
BAC ID319L8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF86,191,82686,191,849AGGGCAGCATCCTCTACAATCTAC
TUP86,191,85086,191,872TGATGAGCGCGAAGCAGAAGCAC
TUR86,191,88286,191,900AGCGCACCTCTCGCTCTTC
TDF86,195,30086,195,321CCATAAAGATCCTGTGGTGAGC
TDP86,195,33986,195,364TTCTCCTTTCAGACCTGCCTGGCCTG
TDR86,195,38986,195,408TGCTGGGTTCTCCACTGAAG

PCR Primers

PrimerSequence
SUCACTGCTAGCTCTTCTCTTC
SDGCCTAGTACCACACTCTTAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR109 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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