| Gene | Bhlhe22 |
| Chromosome | 3 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,054,885 | 18,054,903 | CGGCTTTTCGCTCCACACC |
| TUP | 18,054,905 | 18,054,925 | CCGGGCATGGACCTGTCCCTG |
| TUR | 18,054,973 | 18,054,992 | GATCAGCGGGCTCGAAACAG |
| TDF | 18,055,554 | 18,055,573 | AAATCGCCACGCTGCTCCTC |
| TDP | 18,055,575 | 18,055,602 | CCAAGAACTACATCCTCATGCAGGCACA |
| TDR | 18,055,607 | 18,055,625 | AGCGCCGCATTTCCTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TCCGAGACCAGCCTCCCAC |
| SD | CTGTACTTCCTTGAGATCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
