11726 Ercc6l Status: ES cell colonies screened / QC no positives
| Gene | Ercc6l |
| Chromosome | X |
| Deletion Start | 102,156,865 |
| Deletion End | 102,143,019 |
| Deletion Size | 13,847 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,156,943 | 102,156,963 | CAAGGCTTAGCGGAAGTGGAG |
| TUP | 102,156,920 | 102,156,942 | ACTTTGAGCCCACAACTGGCCGA |
| TUR | 102,156,863 | 102,156,882 | AGGCCAGGGACTGCAAGTTC |
| TDF | 102,143,394 | 102,143,412 | TCTCCCTGTGCTCCTGAAC |
| TDP | 102,143,366 | 102,143,391 | TCGTCAAGTGACCCTATGCCTGATCC |
| TDR | 102,143,305 | 102,143,324 | TTTCCCACGAGCTACAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CCAAAACGTAAGGCCACTCT |
| SD | CTAAGCTGGAAGCACAAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
