11727   Rpl19     Status: ES cell colonies screened / QC no positives

GeneRpl19
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:98020
NCBI Gene ID19921
BAC ID292F5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF98,028,46198,028,480GGGCATAGGTGAGAATGGTC
TUP98,028,48298,028,510AGCCTTTCACTCTGCCTCTTAGATGGGTA
TUR98,028,51198,028,530GACAAGTGCTCTACCCAGTG
TDF98,029,43698,029,458GTGACCTGGATGAGAAGGATGAG
TDP98,029,46098,029,482ATCCTGCGCCGGCTTCTCAGGAG
TDR98,029,56298,029,582GCGGATTCCCTGTTCAAACTG

PCR Primers

PrimerSequence
SUCCCAGATCTCAAATGGCTGG
SDGGACTCAGCCACTCTAGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR70 bp++-
TDFTDR147 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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