11728   Psmb6     Status: ES cell colonies screened / QC no positives

GenePsmb6
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:104880
NCBI Gene ID19175
BAC ID186J5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF70,526,01070,526,029GCCTGTCAGCCCATCCTTAC
TUP70,526,03270,526,055TCCTCAGACCACTGTGGCAAGAGA
TUR70,526,08670,526,110TGGAAACCACAGAATTAGGAACAAG
TDF70,527,31270,527,330TCACTGCCAATGGTGAGAC
TDP70,527,33170,527,353TCTGTGCTTCCACGCCTGTGAAC
TDR70,527,38470,527,407GAGTTAGTAGCTGAACGTGAGGAC

PCR Primers

PrimerSequence
SUCAAATCTTTGAGAAGAGGCTC
SDCATAATGCAACCAGCCATCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR101 bp++-
TDFTDR96 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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