11733 Cd36 Status: Cancelled
| Gene | Cd36 |
| Chromosome | 5 |
| Deletion Start | 17,828,891 |
| Deletion End | 17,811,189 |
| Deletion Size | 17,703 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,828,846 | 17,828,868 | CATTGCTGGAGCTGTTATTGGTG |
| TUP | 17,828,821 | 17,828,845 | CAGTCCTGGCTGTGTTTGGAGGCAT |
| TUR | 17,828,799 | 17,828,819 | CATGTCTCCGACTGGCATGAG |
| TDF | 17,811,452 | 17,811,476 | GCTTGCCATTTAGCTGTTTAAGTTC |
| TDP | 17,811,420 | 17,811,448 | TCTGTCCTCTTAAGTTACCCAGTACTTCC |
| TDR | 17,811,337 | 17,811,356 | GAGGTGCTGACAGCAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGCCACACTGTCGTAAGGAG |
| SD | CTACTTGTTGAATGACAAATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 140 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
