| Gene | Ccdc163 |
| Chromosome | 4 |
| Deletion Start | 116,709,021 |
| Deletion End | 116,714,974 |
| Deletion Size | 5,954 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,709,309 | 116,709,331 | TCAGAGCCTCCTTTCTTTCAGTG |
| TUP | 116,709,341 | 116,709,369 | ACAGCTCACAGTTCTGTACAAATCCCAGC |
| TUR | 116,709,391 | 116,709,411 | GAGCAGGGTCTGTCTGTGTAG |
| TDF | 116,714,842 | 116,714,865 | CTCCAGAACAACTCCCTTCCTAAC |
| TDP | 116,714,870 | 116,714,894 | AACCCAGCAGCTCTCAGTTCCATGC |
| TDR | 116,714,895 | 116,714,915 | TCCTCTTGCCCTAGACTCCAG |
PCR Primers
| Primer | Sequence |
| SU | GATTTGTGGAGGTCTCAACC |
| SD | CTACTCACCAAACAAGAGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11735A-B2 | VGB6 | C57BL/6NTac | |
| 11735A-B5 | VGB6 | C57BL/6NTac | |
| 11735A-B10 | VGB6 | C57BL/6NTac | |
| 11735A-D8 | VGB6 | C57BL/6NTac | |
| 11735A-H1 | VGB6 | C57BL/6NTac | |
