11736   Bola1     Status: ES cell colonies screened / QC positives

GeneBola1
Chromosome3
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1916418
NCBI Gene ID69168
BAC ID171H14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFACCAGTGGAAGCCGCTATTC
TUPCGCCAAATTGGAGCAAGCCCTGA
TURCCACGCGAAAATGCGTCTC
TDFACGAGGCACTGTCGGAGGAG
TDPCTGGACCGGTACATGCCCTGGC
TDRTGTCCAACTGTGGGTTTTCTC

PCR Primers

PrimerSequence
SUCAGCCAAAAGGACCAAGTTG
SDCACTAATGATGTGTAGCTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11736A-D5VGB6C57BL/6NTac
11736A-D7VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image