| Gene | Urod |
| Chromosome | 4 |
| Deletion Start | |
| Deletion End | |
| Deletion Size | |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 116,993,792 | 116,993,811 | TGCAAGCTGGTCTGGAGTAG |
| TUP | 116,993,765 | 116,993,790 | CAGATAACGGCCTGACTGGACCGGTG |
| TUR | 116,993,706 | 116,993,728 | CAGGTGAAAGGTACAGGTAGGAG |
| TDF | 116,990,346 | 116,990,365 | CCCTGACATGGACCCAGAAC |
| TDP | 116,990,318 | 116,990,344 | TGTAGGAGCCTTTGTGGATGCTGTACA |
| TDR | 116,990,295 | 116,990,313 | GTCGAAGCAGGCGTGAATG |
PCR Primers
| Primer | Sequence |
| SU | GTCTTCTATACCACAGATGAG |
| SD | ACTATGCAGAGGAAGCATAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
