| Gene | Prm1 |
| Chromosome | 16 |
| Deletion Start | 10,796,728 |
| Deletion End | 10,796,435 |
| Deletion Size | 294 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,796,691 | 10,796,709 | GCAGCAAAAGCAGGAGCAG |
| TUP | 10,796,666 | 10,796,686 | CGCCGTCGCAGGCGAAGATGT |
| TUR | 10,796,606 | 10,796,625 | CAGCCCAGCCCTCTACTTAC |
| TDF | 10,796,546 | 10,796,566 | GAGTCCCTCTCACCACTTTTC |
| TDP | 10,796,520 | 10,796,543 | ACCTTTCTAGGATGCTGCCGTCGC |
| TDR | 10,796,444 | 10,796,464 | TCTCACGCAGGAGTTTTGATG |
PCR Primers
| Primer | Sequence |
| SU | ATCCATGTGGCTGTTTCACC |
| SD | GGCCCTCATCTCTGACATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11738A-G2 | VGB6 | C57BL/6NTac | |
| 11738A-G4 | VGB6 | C57BL/6NTac | |
| 11738A-H7 | VGB6 | C57BL/6NTac | |
