11741   Ndn     Status: ES cell colonies screened / QC no positives

GeneNdn
Chromosome7
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:97290
NCBI Gene ID17984
BAC ID328M6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF62,348,80562,348,826TGGGCAGCTACAAGAAATGGTG
TUP62,348,82862,348,851AGAAGCATCCTCAGGCGCACCAGC
TUR62,348,85262,348,872GAACACTCTGGCGAGGATGAC
TDF62,349,07762,349,094AGGCCCTGGAAGAAGCAC
TDP62,349,09562,349,117TCCACCTTCGGAGACGTGAGGAA
TDR62,349,17562,349,193GTACTCGGGAGGCTCGATG

PCR Primers

PrimerSequence
SUTACATAAGCCTAGTGGTACC
SDTTTAGCACAGCACATCTCTTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR68 bp++-
TDFTDR117 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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