Gene | Hcrt |
Chromosome | 11 |
Deletion Start | 100,762,313 |
Deletion End | 100,761,263 |
Deletion Size | 1,051 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 100,762,604 | 100,762,624 | TTCCGATGTTCTCTGTGCTTG |
TUP | 100,762,569 | 100,762,596 | TGTCTTGCACTCAACAGGGTAGTAGGGA |
TUR | 100,762,534 | 100,762,554 | AGCCCTTCCTCACCTACAAAC |
TDF | 100,762,034 | 100,762,053 | CCTGCCGTCTCTACGAACTG |
TDP | 100,762,013 | 100,762,033 | TTGCACGGAGCTGGCAACCAC |
TDR | 100,761,984 | 100,762,005 | CGCTTTCCCAGAGTCAGGATAC |
PCR Primers
Primer | Sequence |
SU | ATTCTTTGGGTCCAACCTGG |
SD | ACTGCAAGGTTCCTTGCTGG |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 91 bp | + | + | - |
TDF | TDR | 70 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
11744A-C9 | VGB6 | C57BL/6NTac | |
11744A-D11 | VGB6 | C57BL/6NTac | |
11744A-H1 | VGB6 | C57BL/6NTac | |