| Gene | Zc3h10 |
| Chromosome | 10 |
| Deletion Start | 128,545,558 |
| Deletion End | 128,544,254 |
| Deletion Size | 1,305 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 128,545,347 | 128,545,369 | GCCATCTGTAGAGACTTCCTGAG |
| TUP | 128,545,322 | 128,545,345 | AACGTCTGCAAACGAGGCAAGCGA |
| TUR | 128,545,298 | 128,545,317 | CATGTCTGGGTGGCGGTATC |
| TDF | 128,544,302 | 128,544,321 | CTGTGTCTGTGGCTCCTGTG |
| TDP | 128,544,278 | 128,544,300 | CTCCTGTGGCTGTGTCCATGGCC |
| TDR | 128,544,243 | 128,544,264 | GTGGTGTGGCTCATTGTGATTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCAAGTGTGAATCACTCC |
| SD | TTCTCTCTGCCGGCTCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 295 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 443 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11745A-C8 | VGB6 | C57BL/6NTac | |
| 11745A-C9 | VGB6 | C57BL/6NTac | |
| 11745A-H6 | VGB6 | C57BL/6NTac | |
| 11745A-E2 | VGB6 | C57BL/6NTac | |
