11746   Aarsd1     Status: Cancelled

GeneAarsd1
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1916934
NCBI Gene ID73635
BAC ID323C9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF101,416,749101,416,771CCTTCTGGACAGCTTGGTAGTTG
TUP101,416,724101,416,748TCTGTGAAGGGAAGGGATGAGCCAA
TUR101,416,668101,416,688GCACAGCTCTTCTACCTGAAC
TDF101,407,039101,407,061GCCAGACTATCTCCATAACCATC
TDP101,407,007101,407,034CATTCTCTAGGGTGGCTGAAGTCTTGGA
TDR101,406,983101,407,003CCCTTCTTCCCTGCTCCTTTG

PCR Primers

PrimerSequence
SUACGTCTTAGCTCCAGTTGTG
SDCAAGGAGATTTGGATGAGGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR104 bp++-
TDFTDR79 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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