11747   Psmb3     Status: ES cell colonies screened / QC no positives

GenePsmb3
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1347014
NCBI Gene ID26446
BAC ID351J21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,703,98997,704,007CCACCTTCACCCAGGTTTC
TUP97,704,00997,704,032TCGTTGAGCAGCCCGATATCAGCC
TUR97,704,07697,704,096ACCCATCTCCGTAACGCTGTG
TDF97,713,03097,713,047AGCCAGACCTGAGCTACC
TDP97,713,05197,713,072TGCCCTCTCCCTGTTTGAGGCT
TDR97,713,07397,713,093CAGGACCCACGAGGAAATGAC

PCR Primers

PrimerSequence
SUCTTCCGCACTCACAGTGAC
SDCACAATGACTGAGTTCTACG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR108 bp++-
TDFTDR64 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image