11751   Gale     Status: ES cell colonies screened / QC no positives

GeneGale
Chromosome4
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1921496
NCBI Gene ID74246
BAC ID54D1
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 4

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,965,375135,965,395ATCGGCAGCCACACGGTATTG
TUP135,965,399135,965,421CTGCTGGAGGCAGGCTACTCACC
TUR135,965,454135,965,473TACCCAGCTTCACTCACCAC
TDF135,967,473135,967,490TCTCTTCCAGCCGCTACC
TDP135,967,492135,967,517TGAACCTCAGTGCTAGGTGCCTATGA
TDR135,967,529135,967,547GGGCCTCAGGAACCACAAC

PCR Primers

PrimerSequence
SUCTCTGTCAACAGGAACTAGAC
SDTCACATGAGCTGCTGAACTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR99 bp++-
TDFTDR75 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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