| Gene | Dscr3 |
| Chromosome | 16 |
| Deletion Start | 94,525,256 |
| Deletion End | 94,497,840 |
| Deletion Size | 27,417 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,526,090 | 94,526,112 | TGGTCAATCCTAAAGCAGAGTTC |
| TUP | 94,526,065 | 94,526,088 | CTTGTAAGCCTCTCACCGCGGCAG |
| TUR | 94,526,029 | 94,526,050 | CAGGGCGATTGCATTCTACAAC |
| TDF | 94,499,168 | 94,499,189 | CCTGGGTCAGAAGCACAGAATC |
| TDP | 94,499,144 | 94,499,166 | CAAGCAGCACCTGGACACTGGCT |
| TDR | 94,499,109 | 94,499,127 | CACACCGAGCACTGGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GAAGGCAGCCTTCCTGTG |
| SD | AGACACACTGCTTAATGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11752A-A7 | VGB6 | C57BL/6NTac | |
| 11752A-F2 | VGB6 | C57BL/6NTac | |
| 11752A-B2 | VGB6 | C57BL/6NTac | |
