11753 Prnd Status: ES cell colonies screened / QC no positives
| Gene | Prnd |
| Chromosome | 2 |
| Deletion Start | 131,953,019 |
| Deletion End | 131,953,556 |
| Deletion Size | 538 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,953,179 | 131,953,198 | ACCGCCCAGGAGCCTTCATC |
| TUP | 131,953,202 | 131,953,224 | CAAGGCCGGAAGCTGGACATCGA |
| TUR | 131,953,240 | 131,953,262 | AGTTAGCCGCGTAGTACCTGTTG |
| TDF | 131,953,400 | 131,953,418 | AAGCTCCACCAGCGAGTCC |
| TDP | 131,953,421 | 131,953,444 | TGGCGGCTGATCAAAGAGATCTGC |
| TDR | 131,953,454 | 131,953,473 | TCCAGCCAGAAATCGCAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCTTATGCATCTGTGTTCC |
| SD | AACAGGCCAGGTCTCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
