11754 Scaf1 Status: ES cell colonies screened / QC no positives
| Gene | Scaf1 |
| Chromosome | 7 |
| Deletion Start | 45,016,057 |
| Deletion End | 45,005,634 |
| Deletion Size | 10,424 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,013,249 | 45,013,266 | TCAGCAGGCTGTGGGAAG |
| TUP | 45,013,222 | 45,013,247 | TCCCTTCAGGGAGACCTGCCTAATGA |
| TUR | 45,013,171 | 45,013,190 | AGCCCAGAGGAAGGCAGAAG |
| TDF | 45,003,321 | 45,003,343 | ACCCTGTCAAGGTGAGCAATCTG |
| TDP | 45,003,296 | 45,003,317 | AGGGCCTACGTCCAGCGCTACC |
| TDR | 45,003,275 | 45,003,294 | GACCGTGCTTGCGGAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | AGGTGTTGGAGCAGGTGG |
| SD | CGGTCTTTGAACTGACAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
