11755 Armcx1 Status: Cancelled
| Gene | Armcx1 |
| Chromosome | X |
| Deletion Start | 134,720,154 |
| Deletion End | 134,721,525 |
| Deletion Size | 1,372 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,720,651 | 134,720,675 | GACAAGTGGGAAAGTCAAGAGAAAG |
| TUP | 134,720,679 | 134,720,702 | CGAAGCAAGAGCAACAAGGCTCCA |
| TUR | 134,720,716 | 134,720,735 | GAACTTGCCCTTGCGTACAG |
| TDF | 134,721,044 | 134,721,062 | CATGGTTTGCCGCTTGGAC |
| TDP | 134,721,063 | 134,721,083 | TCAGCCGTCCAGATGGCTGGA |
| TDR | 134,721,164 | 134,721,188 | GGTGAAGTGGTTTCCCAGGAATAAC |
PCR Primers
| Primer | Sequence |
| SU | TTAGAGAATCTGGCTGTTTGG |
| SD | TTGGCATACTTAAAGTGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 145 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
