11757 Nrip1 Status: ES cell colonies screened / QC no positives
| Gene | Nrip1 |
| Chromosome | 16 |
| Deletion Start | 76,372,729 |
| Deletion End | 76,369,247 |
| Deletion Size | 3,483 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,294,194 | 76,294,217 | CAGCAGATTAGACAGAGCCTCAAG |
| TUP | 76,294,168 | 76,294,192 | AGCAGGGATATGCCCTCAGTCACGA |
| TUR | 76,294,130 | 76,294,152 | CGCCATAGCACCTTAAATCCTTC |
| TDF | 76,292,015 | 76,292,037 | TGCCGTAGATACTGCCAATCATC |
| TDP | 76,291,991 | 76,292,013 | CAGCGGCCCGGAAGTACTCTACG |
| TDR | 76,291,901 | 76,291,922 | CACCATCGCTGGCTGAACTATG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCTAAGGAAGTTGCGAG |
| SD | GCTAGTCACAGTTGTTATCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 137 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
