11759   1700027D21Rik     Status: ES cell colonies screened / QC positives

Gene1700027D21Rik
Chromosome10
Deletion Start76,025,114
Deletion End76,032,753
Deletion Size7,640
Genome Build37
MGI IDMGI:1923823
NCBI Gene ID76573
BAC ID164C15
BAC LibrarybMQ
CassetteZEN-UB1rev
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFGGCTATGCTGGTTGGTAATGTC
TUPTCAAAGCACAGCCACCTGGCTTAA
TURAGCCTGGAGCCCTGGAATCAC
TDFGCTTTCCTGACCTGGTTTCTTG
TDPTTTCCTCTGCTCCGTGTCCCACAG
TDRTCACGCAGCTTCTTCTCATCC

PCR Primers

PrimerSequence
SUAGCCGGTGTTATGAACACTC
SDGGTTCTGCTCAGAAACACCA
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11759A-A2VGB6C57BL/6NTac
11759A-A6VGB6C57BL/6NTac
11759A-C5VGB6C57BL/6NTac
11759A-D7VGB6C57BL/6NTac
11759A-F11VGB6C57BL/6NTac
11759A-G10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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