11764 Rg9mtd1 Status: ES cell colonies screened / QC no positives
| Gene | Rg9mtd1 |
| Chromosome | 16 |
| Deletion Start | 56,037,036 |
| Deletion End | 56,035,795 |
| Deletion Size | 1,242 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,034,783 | 56,034,801 | GGGCCAGGTTGCTAGAGAC |
| TUP | 56,034,754 | 56,034,782 | CACCGCAGAAGAACAACAGCAAGACTTCA |
| TUR | 56,034,721 | 56,034,744 | TGTTGATCTGTCGATCCCATAGTC |
| TDF | 56,034,160 | 56,034,181 | TGGGAAGAGGCTCTCAAGTTTG |
| TDP | 56,034,142 | 56,034,159 | TTCCCAGGAGAAAGCATA |
| TDR | 56,034,097 | 56,034,119 | TCCTAACAAGTTCCTGAGATTGC |
PCR Primers
| Primer | Sequence |
| SU | AAGCCATGGTAGCTGAGAAG |
| SD | ATACGTCTAGTCTGGCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
