11765 Pax8 Status: Cancelled
| Gene | Pax8 |
| Chromosome | 2 |
| Deletion Start | 24,475,094 |
| Deletion End | 24,444,571 |
| Deletion Size | 30,524 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,474,857 | 24,474,878 | CTGTTCGACTCTGCTGTCCTTC |
| TUP | 24,474,835 | 24,474,856 | CCCGGCCTTGGCTTCTGAGAAC |
| TUR | 24,474,807 | 24,474,830 | CACAAATAATCCCTCACTGCTTCC |
| TDF | 24,444,916 | 24,444,937 | TGGGCTGAGCAGTTTATGTTAC |
| TDP | 24,444,885 | 24,444,912 | TGCCAAGCTCCTAGAGGATTGTTTCTGA |
| TDR | 24,444,865 | 24,444,884 | CCACTTGCACCCTGTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | GCTGGAAACAGCTGGGTAAG |
| SD | CACCACGTTGAGTCTTACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
