11767 Slc25a19 Status: ES cell colonies screened / QC no positives
| Gene | Slc25a19 |
| Chromosome | 11 |
| Deletion Start | 115,627,753 |
| Deletion End | 115,618,801 |
| Deletion Size | 8,953 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,623,898 | 115,623,917 | ACACGTTCCAGCCCAGATTC |
| TUP | 115,623,872 | 115,623,895 | TCCATAGGCTACGGAGCTGTCCAA |
| TUR | 115,623,834 | 115,623,855 | CCCAAGCACCCAACCAGATTAG |
| TDF | 115,615,463 | 115,615,483 | TCCACTGGCTTCATGTTCTTC |
| TDP | 115,615,434 | 115,615,462 | TGGTACGAGCTCTTCTGTAACCTCTTCCA |
| TDR | 115,615,394 | 115,615,415 | CCTCCTGCCTCACAACTATCTG |
PCR Primers
| Primer | Sequence |
| SU | CTGTTACTGGATGAGGAAGG |
| SD | GGATGGCCAGTTCCTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
