11776 Tceal3 Status: ES cell colonies screened / QC one positive
| Gene | Tceal3 |
| Chromosome | X |
| Deletion Start | 136,667,540 |
| Deletion End | 136,668,145 |
| Deletion Size | 606 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,667,605 | 136,667,629 | GCCTGAAGATGAAGAAAAGTCAGAC |
| TUP | 136,667,631 | 136,667,656 | AGGACGAGAAGCCAGACAAGAAAGCA |
| TUR | 136,667,694 | 136,667,714 | GCCCTTGCTCATCTGGCTTTG |
| TDF | 136,667,921 | 136,667,941 | CAGGACAGGCATGTAAGCAGT |
| TDP | 136,201,345 | 136,201,373 | ATGAGAGAGTGCGCAGATATGACTAGGGC |
| TDR | | | CCCCTGACTCCCCGAGG |
PCR Primers
| Primer | Sequence |
| SU | CTGAAGGAGCACCAAGAAGG |
| SD | ACCATAGAGATGTAGTTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
