11782 Snrpd2 Status: ES cell colonies screened / QC no positives
| Gene | Snrpd2 |
| Chromosome | 7 |
| Deletion Start | 19,149,896 |
| Deletion End | 19,152,740 |
| Deletion Size | 2,845 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,150,137 | 19,150,159 | GGAACCATAAAGGGAAAGCTGAG |
| TUP | 19,150,160 | 19,150,184 | TCTGATGGCACATGCCTGGAATCCC |
| TUR | 19,150,204 | 19,150,223 | GCCAACTAGCGAACCTCCTG |
| TDF | 19,152,000 | 19,152,023 | CAAGCAAGCATTTGTGGAAAGAAC |
| TDP | 19,152,027 | 19,152,051 | TCCAAGTGTGAACAATGGCCACCCG |
| TDR | 19,152,090 | 19,152,107 | AGACACGGCTCCCAACAC |
PCR Primers
| Primer | Sequence |
| SU | CGATTGGTTTACCTGAAGCC |
| SD | TGTAAAGAGGGCAGAGACTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
