11784 Defb14 Status: ES cell colonies screened / QC no positives
| Gene | Defb14 |
| Chromosome | 8 |
| Deletion Start | 19,194,360 |
| Deletion End | 19,195,232 |
| Deletion Size | 873 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,194,567 | 19,194,587 | GAGCCTGAAAGCAGGTTTGTG |
| TUP | 19,194,588 | 19,194,608 | TCTGTCCTGTAACACTCTTAA |
| TUR | 19,194,611 | 19,194,633 | GGCATCTGTGGCAGTATTTAGTC |
| TDF | 19,195,109 | 19,195,129 | GAATAAGAGGTGGCCGGTGTG |
| TDP | 19,195,141 | 19,195,170 | TGCCTTGGAAAGGAAGAACAAATAGGTCGA |
| TDR | 19,195,172 | 19,195,196 | CAGCATTTTCGACCGCTATTAGAAC |
PCR Primers
| Primer | Sequence |
| SU | TTCAAAGGCAATTCAGTCCC |
| SD | GGGAGAAGGCCAGAAAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
