11790 Sf3b4 Status: ES cell colonies screened / QC no positives
| Gene | Sf3b4 |
| Chromosome | 3 |
| Deletion Start | 96,172,577 |
| Deletion End | 96,177,215 |
| Deletion Size | 4,639 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 96,172,789 | 96,172,808 | GCTCCCTCCACCAAGAAGAC |
| TUP | 96,172,818 | 96,172,837 | CCGGGAGTACCGGACCAGCA |
| TUR | 96,172,870 | 96,172,889 | TTCCCTCTGCCACCATCATC |
| TDF | 96,176,839 | 96,176,860 | TGCCTTTGTGCAGCTTTCTTTG |
| TDP | 96,176,864 | 96,176,884 | CTCGCCCTGTCTGGTGCCCTT |
| TDR | 96,176,909 | 96,176,933 | TAATGTGGAGATGAGTAGGGATGAG |
PCR Primers
| Primer | Sequence |
| SU | CAACTCTGCGTCACAGAACG |
| SD | GACACTTCCCAGCCAAATTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
