11791 Ngfrap1 Status: Cancelled
| Gene | Ngfrap1 |
| Chromosome | X |
| Deletion Start | 136,271,274 |
| Deletion End | 136,271,662 |
| Deletion Size | 389 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,271,301 | 136,271,323 | TCCACCAGGAAAACGAAGAGATG |
| TUP | 136,271,325 | 136,271,346 | AGCAGCCCCTGCAGAATGGACA |
| TUR | 136,271,411 | 136,271,432 | GGTGGTTGTGGTTATGGTTGTG |
| TDF | 136,271,498 | 136,271,519 | AATGACGGGTTGGGTGGAGATG |
| TDP | 136,271,537 | 136,271,564 | TTCATGGAGGAGATGAGAGAGATCCGGA |
| TDR | 136,271,589 | 136,271,613 | CCCATAAGGATGCGTAGACAATTTC |
PCR Primers
| Primer | Sequence |
| SU | AAAGCTCAGGGAAAGGATCC |
| SD | TGCTTTATTGACTTGACATTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
