11793 Tmed5 Status: ES cell colonies screened / QC one positive
| Gene | Tmed5 |
| Chromosome | 5 |
| Deletion Start | 108,127,367 |
| Deletion End | 108,119,485 |
| Deletion Size | 7,883 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,131,722 | 108,131,741 | ATTCCCGTGGCAGAGGCAAG |
| TUP | 108,131,696 | 108,131,721 | TGGATCTCTGAACAGTCAAGGCTGCA |
| TUR | 108,131,672 | 108,131,693 | TACTCCCAAGACTGGGTTTCAC |
| TDF | 108,124,746 | 108,124,769 | TCATCTCCAGGAATCCATCAACAG |
| TDP | 108,124,716 | 108,124,743 | TCAAGTCCAGACTAAGCAAAAGTGGCCA |
| TDR | 108,124,628 | 108,124,650 | AACCACAGACCAGAAATTGACTC |
PCR Primers
| Primer | Sequence |
| SU | GTCACCTCGGACTACATTTC |
| SD | TAAAAGCAAACTGGTGGCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 142 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
