11797 Rpl35a Status: Cancelled
| Gene | Rpl35a |
| Chromosome | 16 |
| Deletion Start | 33,057,171 |
| Deletion End | 33,060,141 |
| Deletion Size | 2,971 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,057,272 | 33,057,293 | GGTCTGCTGTAGATAGCTGGAG |
| TUP | 33,057,300 | 33,057,328 | TTGCTACAAAGCAAATCATGGTGGCTTGC |
| TUR | 33,057,380 | 33,057,399 | GCACCACAGCCTAAGACAAC |
| TDF | 33,059,529 | 33,059,550 | GTCAGAGGGCAATTTGTAGGAG |
| TDP | 33,059,551 | 33,059,576 | TCAGCTATTCTGCTGTGGCATTTGCA |
| TDR | 33,059,578 | 33,059,600 | CCTGACCACATGGAGTTTGATTC |
PCR Primers
| Primer | Sequence |
| SU | ATTCCGAGTCGTTCGTTGTC |
| SD | AATCACGCTCCTATAGGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
