11800 Armcx2 Status: ES cell colonies screened / QC no positives
| Gene | Armcx2 |
| Chromosome | X |
| Deletion Start | 134,808,841 |
| Deletion End | 134,806,489 |
| Deletion Size | 2,353 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,806,278 | 134,806,298 | ACCTTCGCCAACAATAGTGTC |
| TUP | 134,806,256 | 134,806,277 | TACTGGGCCAGCAGCAATTCCC |
| TUR | 134,806,176 | 134,806,195 | GCTGGCACAAGAGATTGGAC |
| TDF | 134,804,788 | 134,804,811 | CGGACATGCTAAAGAAACTTCTCG |
| TDP | 134,804,759 | 134,804,785 | ACCCAGGTGCCGTCATCATTTAGTTCC |
| TDR | 134,804,669 | 134,804,690 | GAACACTTCTGCTCTGAGGTTG |
PCR Primers
| Primer | Sequence |
| SU | TAGAGTTGAAGATACAGCCTG |
| SD | CACGAACAGGATTGCTATTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 143 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
