11801 Bet1 Status: ES cell colonies screened / QC no positives
| Gene | Bet1 |
| Chromosome | 6 |
| Deletion Start | 4,085,951 |
| Deletion End | 4,077,039 |
| Deletion Size | 8,913 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,086,698 | 4,086,719 | CAGGGTTGTGGGAGGAGACTAC |
| TUP | 4,086,672 | 4,086,697 | CGGCCCTCTGTCAATGACTTTGGTGT |
| TUR | 4,086,613 | 4,086,633 | TCGGAACTAGGACACAGGAAG |
| TDF | 4,078,737 | 4,078,760 | CAGAGCCCCAATGTGCATTATCTC |
| TDP | 4,078,705 | 4,078,733 | TCGGTGTCTAGTCTGCTCATCTTAAAGGC |
| TDR | 4,078,659 | 4,078,679 | TGTTGACGAGGCACCAAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TTCCAGGCATTGTTCCTACC |
| SD | GGAACAGCAAGGTATGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
