11808 Napsa Status: ES cell colonies screened / QC no positives
| Gene | Napsa |
| Chromosome | 7 |
| Deletion Start | 44,572,619 |
| Deletion End | 44,586,818 |
| Deletion Size | 14,200 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,573,067 | 44,573,085 | GGGTCCCCAACTCTCAAAC |
| TUP | 44,573,086 | 44,573,112 | TGGGTTTCCACCCTGAGAAATTCAGCA |
| TUR | 44,573,131 | 44,573,153 | CCTTGCTTCCTTCCCATTACTTC |
| TDF | 44,586,721 | 44,586,742 | CGTGCTCAGTCTCGTTCAACAG |
| TDP | 44,586,744 | 44,586,768 | CCGGGCAGAAAGAAGGACTACGCAG |
| TDR | 44,586,794 | 44,586,814 | CCGGTGAGCTTGTACCCTAAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCCTGTTCCTCCCTCTTG |
| SD | ACGCTTGTTGTGGCTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
