11813 Irs1 Status: ES cell colonies screened / QC no positives
Gene | Irs1 |
Chromosome | 1 |
Deletion Start | 82,241,409 |
Deletion End | 82,237,536 |
Deletion Size | 3,874 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 82,290,008 | 82,290,029 | CAAGGAGGTCTGGCAGGTTATC |
TUP | 82,289,978 | 82,290,001 | CCCAAGGGCCTGGGTCAGACAAAG |
TUR | 82,289,947 | 82,289,966 | TCAGGCAGAGGCGGTAGATG |
TDF | 82,287,251 | 82,287,275 | CAACCATAACCAGAGTGCCAAAGTG |
TDP | 82,287,227 | 82,287,249 | TTCGCGCAGACACTCAAGGGTGC |
TDR | 82,287,182 | 82,287,203 | AGCCCGAGTAGGTGCTGAGAAG |
PCR Primers
Primer | Sequence |
SU | TCCCTCTGGGCATGAAACG |
SD | TCAGAACTCTGAGAAAGGATC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 83 bp | + | + | - |
TDF | TDR | 94 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |