| Gene | Eln |
| Chromosome | 5 |
| Deletion Start | 134,747,238 |
| Deletion End | 134,718,026 |
| Deletion Size | 29,213 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,746,998 | 134,747,015 | TCGAGACAGGGAGGGTTC |
| TUP | 134,746,968 | 134,746,990 | TGTCTCCTCTTGGGAGCCAGCTC |
| TUR | 134,746,932 | 134,746,950 | TGGCGGGAGTGTAGCTTTG |
| TDF | 134,718,184 | 134,718,205 | CAAGGCTCAGTCCAGGACAATC |
| TDP | 134,718,157 | 134,718,182 | TCTTTCCATCTACAGGAGCTGCTGGA |
| TDR | 134,718,136 | 134,718,155 | TGGTCCACCAGGCACTAAAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGCTACAGAGGGAAAGCG |
| SD | ATCTACCACTTACTGAGTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11817A-B8 | VGB6 | C57BL/6NTac | |
| 11817A-D10 | VGB6 | C57BL/6NTac | |
| 11817A-E6 | VGB6 | C57BL/6NTac | |
| 11817A-F7 | VGB6 | C57BL/6NTac | |
| 11817A-H4 | VGB6 | C57BL/6NTac | |
| 11817A-C3 | VGB6 | C57BL/6NTac | |
