11819 Ccdc23 Status: ES cell colonies screened / QC positives
| Gene | Ccdc23 |
| Chromosome | 4 |
| Deletion Start | 119,195,707 |
| Deletion End | 119,201,037 |
| Deletion Size | 5,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,195,732 | 119,195,753 | TGAGACTCAGAAGTGAGGAGAC |
| TUP | 119,195,754 | 119,195,777 | TCGACTAGGAACGCACAGCAAGGT |
| TUR | 119,195,834 | 119,195,854 | GAACCGCTTGCTAGTCCTTTC |
| TDF | 119,200,930 | 119,200,950 | GAGCAGCAGCAGTTTGATGAG |
| TDP | 119,200,953 | 119,200,975 | CTGTAAGCAGATGCAGCCGCCTG |
| TDR | 119,201,007 | 119,201,025 | CAAAGGCAGGCACCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGTCTACGACGTGGCGGA |
| SD | TTCTTTGTTCCACTTTCTGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
